University Of Arkansas Developing Aflatoxin-Resistant Corn

University of Arkansas Division of Agriculture plant pathologists are developing transgenic corn lines that could help level the field for corn producers in the South compared to those in the Midwest where a cooler climate eliminates the risk of a major fungal disease that plagues Southern growers.

A major issue for corn in the South is infection by a fungus that produces aflatoxin in the grain, which makes it unmarketable. The fungus, Aspergillus flavus, thrives in corn plants that are stressed by hot, dry weather, says research assistant professor Ron Sayler. Aflatoxin is rarely a concern for corn producers in the Midwest because of the cooler climate there.

In Arkansas, irrigation is highly recommended for corn to reduce the risk of aflatoxin contamination; however, it frequently occurs even in irrigated fields, particularly in a very hot, dry summer such as occurred in 2010, Sayler said. In many third world countries where grain marketing is unregulated, people and farm animals often die from aflatoxin poisoning.

Sayler and assistant professor Burt Bluhm are working to develop transgenic corn lines in which the growth of the A. flavus fungus that produces aflatoxin would be inhibited. The project is funded in part by the Arkansas Corn and Sorghum Promotion Board.

A gene in hyacinth bean has been proven to inhibit the alpha amylase enzyme activity of A. flavus necessary for both fungal growth and the subsequent accumulation of aflatoxins, Sayler said. Plant pathologist Charles Woloshuk isolated the gene about 10 years ago at Purdue University, where Bluhm was a member of the research team before coming to Arkansas. Insertion of the gene into corn was not being pursued, so Bluhm and Sayler picked up the project in Arkansas.

Sayler developed a DNA segment containing the gene and introduced the transgene into corn line Hi II.

Sayler reported that, “the amylase inhibitor gene family member B01 has been cloned into the plant transformation vector 1300S, which is designed to express B01 in all tissues (of the corn plant). Twenty-one lines with the B01 amylase inhibitor transgene have been regenerated and are being grown to maturity in the greenhouse. Seed from each of these lines will be tested for their ability to inhibit Aspergillus flavus infection and aflatoxin accumulation.”

“When you introduce a gene into corn, it goes to different parts of the corn chromosome. Where the gene is located affects the expression of the desired genetic trait,” Sayler said. Tests of seed from each transgenic line will determine which line will significantly inhibit growth of the fungus and production of aflatoxin. Results should be known by this summer, he said.

“I am optimistic that one of the lines will express (amylose inhibitor) levels high enough to inhibit growth of the fungus,” Sayler said. He is also pursuing a different method of expressing the gene in corn to optimize the effectiveness of this technology.

When a transgenic line is identified that significantly inhibits A. flavus growth and aflatoxin development, it will be made available to plant breeders for use in developing improved corn varieties with a combination of traits best suited for Arkansas and other Southern states, Sayler said.

(Source: University of Arkansas news release)

Leave a Reply

Seed/Biotech Stories
Seed/BiotechSyngenta Delivers Two New Winter Wheat Varieties To Eastern Corn Belt
August 6, 2014
Adding to its portfolio of AgriPro brand wheat varieties, Syngenta introduces SY 007 and SY 474 soft red winter wheat varieties. Read More
Seed/BiotechWater Optimized Seed Flowing Freely
April 1, 2014
As the demand for ag to diminish its water use while maintaining high yields and crop quality increases, three companies are leading the movement to grow more with less water. Read More
Seed/BiotechBayer Applies For Seed Treatment That Protects Against Sudden Death Syndrome
February 26, 2014
If approved, ILeVO seed treatment would be the first product to provide breakthrough protection for soybean seedlings from the soil borne pathogen Fusarium virguliforme, the fungus that causes SDS. Read More
Seed/BiotechSeed Still Central
November 1, 2013
Advancing genetics continue to make seed the crop variable that calls the shots for the season. Read More
Top 100 Articles
CropLife 100CHS Partners With Northern Partners Coop On Fertilizer Warehouse
January 21, 2015
In addition, the two companies will form a joint venture to build and operate a grain barge loading facility that will handle corn, soybeans and wheat for export through the CHS terminal at Myrtle Grove, LA. Read More
CropLife 100Wilbur-Ellis Move To Colorado Complete
January 20, 2015
Wilbur-Ellis Co. announces the opening of its new Agribusiness Division location in the Denver, CO, Metro Area. The new facility will host key personnel as the company shifts the base of its Agribusiness operations from California to Colorado. Read More
CropLife 100United Suppliers, McGregor Co., Five Others To Form Aligned Ag Distributors
January 20, 2015
These companies will continue to operate as independently owned and operated businesses, however, their crop protection divisions will now be formally aligned under the umbrella of Aligned Ag Distributors. Read More
CropLife 100Bozeman Named SEEDWAY Chief Operating Officer
January 16, 2015
He succeeds Donald Wertman, who will retire at the end of August 2015 after 40 years with the GROWMARK subsidiary. Read More
CropLife 100Wilbur-Ellis’ Craig Bair Honored For Safety Contributions To Ag Aviation
January 14, 2015
The William O. Marsh Safety Award recognizes significant achievements in safety, safety education or an outstanding operational safety program. Read More
CropLife 100BRANDT Gearing Up For Another NASCAR Season
January 13, 2015
BRANDT will return for its fifth year as a primary sponsor of the No. 51 driven by Justin Allgaier in the NASCAR Sprint Cup Series. Read More
Latest News
ManagementRetail Collaboration, Communicating Agriculture’s Good …
January 23, 2015
Editors Paul Schrimpf and Eric Sfliligoj discuss the recent creation of Aligned Ag Distributors, and the US Farmers and Ranchers Read More
FertilizerOSU Expert: Toledo Water Crisis A Turning Point For Ohi…
January 23, 2015
The August 2014 water crisis in Toledo, OH, impacted Ohioans’ views of Lake Erie algae problems by increasing the attribution of blame of algae growth on agriculture. Read More
FertilizerH.J. Baker Launching TIGER XP
January 22, 2015
Tiger-Sul introduces this next generation sulphur-bentonite product with a proprietary activator – ensuring farmers have a well-balanced nutrient replacement program. Read More
Crop InputsH.J. Baker Expands China Sales Force
January 21, 2015
Global Agricultural firm H.J. Baker announced that sales veteran Shi Dongshen has joined their Shanghai office in China. This newest hire follows the recent opening of their Lianyungang Sulphur Bentonite Plant. Read More
CropLife 100CHS Partners With Northern Partners Coop On Fertilizer …
January 21, 2015
In addition, the two companies will form a joint venture to build and operate a grain barge loading facility that will handle corn, soybeans and wheat for export through the CHS terminal at Myrtle Grove, LA. Read More
Matt Hopkins5 Bold Predictions For Ag Retail In 2015
January 21, 2015
This past year was filled with ups and downs for the nation’s top ag retailers, but what will 2015 have in store? Read More
SoftwareDeere Launches App Center
January 20, 2015
The John Deere App Center provides users the ability to search and find apps most useful to their business or operations. Read More
Crop InputsNew Syngenta Herbicide Approved In Soybeans, Sunflowers
January 20, 2015
Syngenta will sell BroadAxe XC herbicide in 2015 for use on soybeans, sunflowers and dry peas Read More
CropLife 100Wilbur-Ellis Move To Colorado Complete
January 20, 2015
Wilbur-Ellis Co. announces the opening of its new Agribusiness Division location in the Denver, CO, Metro Area. The new facility will host key personnel as the company shifts the base of its Agribusiness operations from California to Colorado. Read More
Crop InputsNovozymes Sales Figures Up For 2014
January 20, 2015
Novozymes announces its sales growth in 2014 was in line with expectations as sales grew by 7% compared with 2013. Read More
CropLife 100United Suppliers, McGregor Co., Five Others To Form Ali…
January 20, 2015
These companies will continue to operate as independently owned and operated businesses, however, their crop protection divisions will now be formally aligned under the umbrella of Aligned Ag Distributors. Read More
Eric SfiligojGMO Or DNA? LOL Or WTF?
January 19, 2015
A new survey finds the American public seems generally confused when it comes to any three-letter descriptor for their food. Read More
FertilizerAGROTAIN Wins No-Till Product Of The Year Again
January 19, 2015
This marks the 10th straight year AGROTAIN nitrogen stabilizer has been named the top product in this category; no other product has matched this honor Read More
FertilizerTFI President To Give Update On Nutrient Management Eff…
January 19, 2015
The Fertilizer Institute President Chris Jahn will give the keynote address at the 2015 Ohio AgriBusiness Association Industry Conference February 4. Read More
Seed/BiotechUnderstanding The Role Of Seed Enhancers In Seed Treame…
January 17, 2015
Seed enhancers such as polymer play a key part in helping to improve germination, seed growth or accuracy of planting. Read More
Crop InputsUSDA Signs Off On Dicamba-Tolerant Trait In Cotton, Soy…
January 16, 2015
A registration decision by EPA for BASF's Engenia herbicide is anticipated to follow the dicamba-tolerant trait deregulation milestone. Read More
CropLife 100Bozeman Named SEEDWAY Chief Operating Officer
January 16, 2015
He succeeds Donald Wertman, who will retire at the end of August 2015 after 40 years with the GROWMARK subsidiary. Read More
Crop InputsDan Clinker Joins Monty’s Sales Team
January 16, 2015
Monty's Plant Food Co. has hired Dan Clinker as a Product Consultant and Sales Support Representative for the Central Ohio region. Read More