More Corn Rootworm Resistance To Bt Corn Reported In Illinois

With the addition of three more counties, a total of five Illinois counties have now reported confirmed cases of field-evolved resistance to Bt corn (Cry3Bb1 protein) by Western corn rootworm, and a University of Illinois entomologist said now is the time to move forward aggressively with integrated pest management (IPM) strategies, according to the Illinois Ag Connection.

In August 2012, in cooperation with an Iowa State University lab, Mike Gray said he confirmed resistance in Henry and Whiteside counties in northwestern Illinois. Working with Joe Spencer of the Illinois Natural History Survey and utilizing single-plant bioassays with larvae collected last summer, Gray said resistance to the Cry3Bb1 protein has now also been confirmed in McDonough, Mercer, and Sangamon counties.

The suspected Bt-resistant larvae collected last summer were exposed to a hybrid expressing the Cry3Bb1 protein as well as its corresponding isoline (not expressing the Cry3Bb1 protein), Gray said. Larvae obtained from three control colonies of Western corn rootworms also were used in the bioassays. The control larvae had never been exposed to corn rootworm Bt protein and were provided by the USDA North Central Agricultural Research Laboratory in Brookings, SD.

Gray said there continues to be some controversy regarding the most appropriate procedures (plant-based vs. diet-based bioassays) that should be used to confirm whether or not resistance to a Bt protein has developed by an insect population. Citing a University of Arizona study that defines key terms regarding resistance to Bt crops and pesticides, Gray offered the following definitions that are useful in communicating with producers who have experienced greater than expected levels of damage to rootworm Bt hybrids in their fields.They include:

  • “Resistance: genetically based decrease in susceptibility to a pesticide”
  • “Field-evolved resistance (= field-selected resistance): genetically based decrease in susceptibility to a pesticide in a population caused by exposure to the pesticide in the field”
  • “Practical resistance (= field resistance): field-evolved resistance that reduces pesticide efficacy and has practical consequences for pest control”

“It has become increasingly evident that some producers have experienced a loss of efficacy with some Bt hybrids in their fields in recent years,” Gray said. “To date, most of those fields have been in continuous corn production, and producers have not rotated traits. It’s also clear that ‘practical consequences’ have resulted due to the loss of efficacy associated with some Bt hybrids in problem fields.

“The primary consequence so far has been an escalation in the use of planting-time soil insecticides with Bt rootworm hybrids. This practice may hasten the onset of resistance evolution to Bt proteins as outlined in a paper published last year,” he added.

Other consequences of the evolution of practical resistance by the western corn rootworm has been the increased use of pyramided Bt hybrids, the use of seed blends as a primary refuge strategy, and a reduction in the size of the required refuge — from 20% to 5% for some products.

“The reduction in the refuge size remains troubling for many entomologists, especially in areas of the Corn Belt where resistance has been confirmed to one of the rootworm Bt proteins. Although a seed blend offers many advantages, including ensured compliance as well as improving the chances that Bt-susceptible and resistant adults will mate, increased selection pressure on the one effective Bt protein within a compromised pyramid, may be a primary consequence of a 5% refuge,” he said.

To date, four Bt rootworm proteins have been registered for use in the United States: Cry3Bb1, Cry34/35Ab1, mCry3A, and most recently eCry3.1Ab. Gray said that Aaron Gassmann (Iowa State University) and his colleagues recently published a paper in which they confirmed the field evolution of resistance to the mCry3A protein. In the same journal article, they also confirmed cross-resistance between Cry3Bb1 and mCry3A proteins.

“These findings are troubling from many perspectives. For instance, pyramided Bt hybrids that are used in areas of the Corn Belt where practical resistance to the Cry3Bb1 and mCry3A proteins is a reality, may in fact be relying to a great extent upon the efficacy of Cry34/35Ab1 or eCry3.1Ab to ensure adequate root protection — again, at reduced refuge size requirements (5%),” he said.

Last summer, Gray reported on the failure of some Bt hybrids (Cry3Bb1) to provide adequate root protection in rotated corn in Kankakee and Livingston counties. Spencer will conduct plant-based bioassays on the offspring reared from the adults collected from these fields. Results from these bioassays will be in later this year.

“If the results confirm resistance to the Cry3Bb1 protein, it seems clear that a segment of the Western corn rootworm population can now overcome rotation and this protein. With cross-resistance within this population to the mCry3A protein a possibility, we begin to see how diminished our IPM tool box is for this insect pest,” Gray explained.

“As I have done in the past, I urge producers to implement a long-term integrated pest management approach for corn rootworms. This includes the use of multiple tactics (over time, not all in the same season), such as: use of a more diverse crop rotation system, use of a non-Bt hybrid in conjunction with a planting-time soil insecticide, rotation of pyramided Bt hybrids, and consideration of an adult suppression program in heavily infested fields,” he said.

Leave a Reply

Crop Inputs Stories

MicronutrientsMicronutrients Going Macro
September 9, 2014
Between 2014’s fantastic growing conditions and a heightened awareness on plant nutrition, the major players in micronutrients are gearing up for another big year. Read More
FertilizerFall Fertility 2014: Forecasting Fertilizer Use
September 7, 2014
Great crops this year have tapped the soil, and fall work is definitely called for, but how challenging will that get? Read More
FungicidesSudden Death Syndrome, Brown Stem Rot Reported In Indiana Soybeans
September 3, 2014
Farmers and retailers should be watching for symptoms of these two diseases over the next few weeks as they are best managed through preventative methods. Read More
HerbicidesScouting Key To Next Season’s Soybean Herbicide Program
August 12, 2014
When growing soybeans, growers need to think ahead to stay one step ahead of weeds. That means examining weed threats and evaluating which herbicides work best. Read More

Trending Articles

Equipment2014 Product Of The Year Voting
September 19, 2014
Many new products were introduced to the ag retail marketplace this year. From this group, CropLife IRON and its consulting partners have selected five finalists for the Product of the Year award. Please cast your vote today to help us determine the winner. Read More
FertilizerFall Fertility 2014: Forecasting Fertilizer Use
September 7, 2014
Great crops this year have tapped the soil, and fall work is definitely called for, but how challenging will that get? Read More
CropLife 100CHS To Build $3 Billion Fertilizer Plant In North Dakota
September 5, 2014
The fertilizer plant in Spiritwood will be the single largest investment in CHS history, as well as the single largest private investment project ever undertaken in North Dakota. Read More
EquipmentNew Holland Acquires Miller-St. Nazianz
September 3, 2014
The assets of Miller acquired as part of the transaction will become part of New Holland Agriculture, a CNH Industrial brand, building on a successful four-year partnership between the two companies. Read More
CropLife 100Pinnacle Acquires East Kansas Chemical
September 2, 2014
Ranked 82nd on the CropLife 100, East Kansas Chemical will operate as part of Pinnacle's Performance Agriculture brand. Read More
MAGIE 2014 ShowStopper
EquipmentJohn Deere Again Wins MAGIE ShowStopper Award
August 25, 2014
For the second consecutive year, John Deere was honored at the Midwest AG Industries Exposition (MAGIE) for its new R4045 sprayer. Read More

Latest News

FertilizerFertilizer Outlook And Technology Conference Set For No…
September 30, 2014
Conference participants can expect to gain perspective on the outlook for agriculture and major fertilizer materials and inputs from industry experts. Read More
MicronutrientsVerdesian Acquires Micronutrient Supplier
September 30, 2014
Verdesian Life Sciences has acquired QC Corp., a producer and supplier of granular and dry micronutrients and ferrous sulfate products. Read More
Eric SfiligojHearing From Young Ag Voices
September 30, 2014
Potential future leaders in ag retail had the chance to shine at the recent Mid America CropLife Association meeting. Read More
WebinarsUpcoming Webinars
September 30, 2014
Register for one of our upcoming Webinars or access our archive of past Webinars to view recordings of presentations that may be of interest to you. Read More
Crop InputsSyngenta Appoints New Chief Operating Officer
September 29, 2014
Jon Parr will succeed retiring COO John Atkin at the end of the year. Read More
CropLife 100South Dakota Wheat Growers To Add Grain-Agronomy Facili…
September 26, 2014
Planned to be constructed in Kennebec, the facility will be located along the soon-to-be rehabilitated line of heavy-rail service from Chamberlain to Presho. Read More
CropLife 100GROWMARK Announces Vice President Appointments
September 25, 2014
Two individuals have been appointed Vice President positions on the GROWMARK executive team. Read More
FertilizerFertilizer Industry Organizations Join Global Alliance …
September 25, 2014
The Fertilizer Institute, the International Plant Nutrition Institute and the International Fertilizer Industry Association are encouraging the industry to promote the proper use of fertilizer products to minimize their environmental impact. Read More
Precision AgARA Organized Precision Forum Hits Capitol Hill
September 24, 2014
The Coalition for the Advancement of Precision Agriculture (CAPA) hosted its inaugural Precision Ag Forum, Sept. 18, in Washington, D.C. Read More
FertilizerFactors To Consider When Placing Fertilizer With Seed
September 24, 2014
The type of crop, fertilizer source, row spacing and soil environment all affect how much fertilizer can be safely applied with seed. Read More
FertilizerSidedressing Manure Into Corn Has Promising Results
September 24, 2014
Applying manure to growing crops can boost yields, reduce nutrient losses and give livestock producers another window of time to apply manure to farm fields. Read More
FertilizerWhen To Sample Manure For Analysis
September 24, 2014
Because the goal is to collect a sample that represents the manure actually being applied, the best time to sample is during loading or field application. Here's why. Read More
Syngenta
Seed/BiotechSyngenta Faces Second Lawsuit Over Agrisure Viptera Cor…
September 23, 2014
A second company has sued Syngenta AG over sales of genetically modified corn seed not approved by China. Read More
StewardshipCover Crops Field Guide For Farmers Expanded
September 23, 2014
Farmers interested in planting cover crops to improve soil health now have an updated and expanded resource in the second edition of the Midwest Cover Crops Field Guide. Read More
HerbicidesArysta LifeScience Launches Contest For Wheat Growers
September 23, 2014
The Flush after Flush Photo Finish contest encourages farmers across the U.S. to participate through Instagram or Twitter using the hashtag #FlushAfterFlush. Read More
FertilizerCF Industries Confirms Merger Of Equals Discussions Wit…
September 23, 2014
CF Industries Holdings, Inc. has confirmed that it is in preliminary discussions with Yara International regarding a potential merger of equals transaction. Read More
StewardshipProgram To Examine Ag-Related Practices, Policies To Pr…
September 22, 2014
A panel discussion on October 14 will include a significant discussion on key issues and solutions related to all aspects of harmful algal blooms. Read More
Eric SfiligojIt’s Product Of The Year Voting Season
September 22, 2014
Readers of CropLife IRON will choose 2014’s best new product over the next month. Read More